The Chapel of the Holy Sepulchre was built within the closed walls of the monastery. It is entered from the gallery through a stone Renaissance portal. The chapel was built on the plan of a square and covered with a lanterned dome. The transition from the square section of the walls into the round dome features corner squinches: an architectural feature that distinguishes the chapel structurally from the ones using the predominant form of arches and pendentives. The Sepulchre of the Lord standing inside assumed the form of a square stone building of a fairly small size, with two storeys divided by pilasters and covered in a full-bodied decoration. The entire construction is currently covered with a wooden baroque superstructure developed late in the 17th century and brought to Miechów from Kraków in 18th/19th century. The entrance to the burial chamber is situated in the front section, on the left-hand side. The chapel provides a powerful backdrop to the liturgies of Paschal Triduum.

Centuries have passed and the historic chapel has regained its original decoration: the Renaissance murals discovered in 2008, with figural, heraldic (coats of arms comprising those of Sigismund the Old, Queen Bona, and the Equestrian Order of the Holy Sepulchre) and vegetable motifs, and imitation caissons with rosettes in the dome. It is highly probable that the colourful murals were painted by a Kraków artist Stanisław Samostrzelnik or members of his workshop, as suggested by the historical circumstances, style of painting, refinement of the form, and mastery of execution.